FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578136
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3655.9016817E-515.9064511
TATAGGC2800.003011490515.5029188
ACTACCT2950.00426186514.7146364
CCTTGAC5551.5393985E-411.7686231
TATTCGA4700.00723731210.7750797
CTCCAAT5600.002186355410.3675971
TCAATTA7753.276019E-49.3350913
CCTTGAT7853.6103718E-49.2449911
ATATTCG6500.00767929068.9042416
CTTCTCA7850.00370697658.3204921
ATCAAAC7900.00392554658.2678311
CCTTTAT10609.3293194E-58.2158321
CTTTGCT8100.0049170558.0636861
CAACATC8250.0057984927.9170741
CTCAAAA8750.0098160357.4646691
CTTTAAT11702.9289542E-47.44340321
CATCCAT9750.0030886717.4434031
CTTCCAT11450.0019010126.97209551
CTTCTAT10900.009120286.658091
CTTTGAT12850.00636319076.212491
Produced by FastQC (version 0.11.5)