FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466364
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAG4500.00498418811.3308091
CTCAGTA4850.00869805910.5131211
CCAAGGT8750.00102868948.3246761
CTTAAAT10900.00108808097.3509181
CCCTGAT11600.00210322376.9073271
CTCCGTA10900.0088211176.68265251
ACGAAAT14404.688158E-46.5115623
CTTAAAG12450.0044169736.43574331
CTTTAAA22804.1429485E-76.3895531
CACAAAG16954.525441E-46.0163581
CAACAAA25454.652502E-76.01044851
CCCAGCA14750.0038936345.926041
GGATTTA18502.4774665E-45.84822234
TTTTACG18652.7650702E-45.8011857
TCATTTG15250.0062875845.6756524
CGATAAT15550.006994755.62116341
CTTTGAC16000.0095768355.46306851
CTTTTGA22901.1835483E-45.40740351
CTTTTGG17750.0050854285.33482741
CTCTTTT29507.0735805E-65.1852851