FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511264
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCC2359.384075E-418.4536423
CTCCCTC2700.002339163316.1008971
CCCTCGA3750.001319451513.5247551
CTCTGAT4350.004020858611.6592711
CTTTATT6708.900996E-49.7326321
ATCAATA7800.00361985678.3420762
CTTTTCG9450.00230971847.66709381
CTTCTTT13703.001644E-57.40406231
CTTCATC11950.0030314966.6694091
TGATTTT14700.0040738715.90014464
CTTTTTC20900.00119876245.2000511
Produced by FastQC (version 0.11.5)