FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212442
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAC7350.00208374048.8694711
CACTCAC9650.00285382947.5061151
AGACCCA10000.00414312357.22502378
CTTAAAC15201.545279E-57.14809371
TAACTAA13200.00122731096.56820344
CTCGAAT14950.00481867465.81410071
CTTTAGC17658.437719E-45.7308976
CTCGCTT16550.00237790845.689681
CTCAATT19153.7436537E-45.6736821
CTAAAAC17950.00101223525.64944931
GATTTAG15450.007133835.6116695
CCCGGAT37851.3897079E-95.5497651
ATTTAAG24901.4288982E-55.51307066
CCCAAAT22054.3566286E-45.25598241
CTTGAAT34952.33209E-75.18125961
CTTCGGA35152.6346243E-75.1517791
CTTGGAT21200.00146391955.12504771
CCCGCTT25801.4053208E-45.05353551
TTCGATT31555.1400802E-65.04016972
CTTCGAT31655.2422765E-65.03490731
Produced by FastQC (version 0.11.5)