FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455958
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGAC2000.00785832318.1454331
CCAAGAG2851.6165085E-417.8270951
ACCGAAT2650.002039995516.43361
CTCCGAG2900.003721227615.01691151
CTACGAT3250.00792289313.3997051
CCCAAAA3900.001749180513.0274911
CTCAAAA5401.1901101E-412.0969561
CACCGCT5059.210813E-411.4980971
CTGCATT8405.9776467E-610.3688191
CTTCAAT6759.371702E-49.6775651
CTTCAAA7502.27888E-49.6775651
ATTTTCC10650.0079553386.75240856
Produced by FastQC (version 0.11.5)