FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555424
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCCT5802.1968405E-411.3294421
CCCTCAC6100.0042304739.5753391
CCCAGGA7200.00160583639.1264951
CTCTGAT11452.4622495E-68.9272271
CCCAATT8457.108533E-48.6404691
CTCAACT7950.0039431558.2655051
CCCCATT8350.00613210777.8695531
CTCAAAG15903.9555198E-77.80631071
GTCCGAA11251.7425072E-47.7879431
CCCGCTT9600.00250721167.60541251
AATTGAG9850.0036363817.3218045
CTCAGAA10100.0041185467.22890761
GTTTAAT11500.00187282546.9837531
CTCCAAC10800.0078903476.76036741
CCCAGTT11000.0094181836.6374511
CCCAAAG14454.2171546E-46.56855061
CTTTAAT24703.7118298E-86.50309041
CTTCTAT19601.0610933E-56.332671
CTTCTGA16352.760518E-46.2517891
CTCTTAT12850.0059925216.2500511
Produced by FastQC (version 0.11.5)