FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912813
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAC4800.007785185210.6707451
CAACAGG5800.002735764710.0925261
AATGACC8306.5865385E-48.7060842
CTTCACG8759.827296E-48.362381
AACCTTG7800.00364787228.3363795
CTCTGCT9803.3162363E-48.2130511
CACCAAT8400.0063393877.83973031
AAGATTC8650.009231217.5171973
GAAGATT13602.8321152E-57.43858052
CCAAGAA10250.00465024737.1386161
CAACAGA10300.0048756317.1039631
CTTAAAA12857.8167435E-46.83307271
CCATGAT10750.00738057876.8065881
CTTCAAG13400.0012578816.5526111
CTTTTGA15207.5751625E-46.2580311
CCTTGAT13000.0066020126.1913771
CTTTTAT18101.5083549E-46.06388041
GAATTTA13250.0091698985.9979984
CAATGAA15450.0061889355.6831711
CTTTATT16850.00260358895.64522651
Produced by FastQC (version 0.11.5)