FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499935
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCC1453.4304865E-431.081175145
CCAAGAT3650.001100789413.853233
CCCGCTT4000.002130553812.6906411
CTACAAC4150.002808472112.2319431
CTTCAAC5450.001754630210.6448361
CACCGAT5500.001893249510.5480651
CTTAAAA8507.782375E-59.3846751
CTTCCAC6200.00510578849.3571541
CTTTAAT9051.5406811E-48.8143371
CTTTCAT10004.7582318E-58.7021541
CTTTTCT14054.9680075E-67.742131
CTTCCAT9500.0024112767.63346861
CTTTTAT13151.4376688E-47.1690751
GCACGTC2100.00666488436.879771100-104
CTTCTTT18253.855739E-66.75509641
CTTCTTC15600.0075838335.57830331
CTTTTTC22951.2985956E-45.37171
Produced by FastQC (version 0.11.5)