FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385730
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAT4850.0086072210.527951
CACGATT7301.6563524E-49.9922811
CTCATGA8104.3196138E-59.9059291
AGATCTC6400.0069762079.01069454
CTTCGAC13151.8845276E-78.8752741
CCCAATT6700.0092157178.709691
CTTCAGA16306.9610905E-88.0551271
CTTACGA8150.00497311778.0551271
CTTTGAT19101.873559E-98.0199831
CTGAAAT20951.6552804E-108.0081341
CTTCGAT14054.5991364E-67.7875791
CTTCGAG11301.8538456E-47.7462291
CTACGAG8550.00764315447.67827941
CTCCGAA12456.8268506E-57.6166061
CTCCAGT9800.00309596517.44323061
GTTTGAT15201.397684E-57.1983871
CTCATCT10400.005524057.01381351
GTCCGAA11450.00180599697.00768761
CTTCTCT15752.3005547E-56.94701531
CTCAAAT11550.0019796736.9470151
Produced by FastQC (version 0.11.5)