FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202521
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4600.031.3772857
CACGTCT5600.027.0629069
ACACGTC6150.023.4691898
AGAGCAC7800.019.4313963
GAGCACA7850.019.307634
AGCACAC7550.019.1172875
GCACACG8450.017.935186
GAAGAGC15200.010.96481
TAACGGG4850.00931598210.4159659
AAGAGCA15553.6379788E-1210.2157512
AAGTCAC6250.00568643459.2374736
AGTCACT4151.6189006E-108.01952520-24
GTTTAAA8350.0065592967.81038671
GTGGAAT11251.9005242E-47.72938971
AATCTTG13954.0356004E-57.24264245
TTCTGAC12200.0039360586.5069296
CAGTCAC6052.382876E-106.45769120-24
CTTCAAC13400.0099955095.94845771
TAAAAAC17155.880408E-45.89443162
ACTGTGA7459.822543E-105.62764925-29
Produced by FastQC (version 0.11.5)