FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542454
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5300.021.704827
ACACGTC5501.09139364E-1018.301118
CACGTCT6501.1805241E-915.4855559
GAGCACA7756.002665E-1114.8437774
GTAGGGT3050.005473114.1726661
GCACACG8503.747118E-912.6873326
AGAGCAC8203.0919182E-812.274343
AGCACAC8856.8266672E-912.1855755
AGGTTAA6250.00586643079.2028449
TTGGCAC12557.304909E-46.87463437
CTCGAAA10650.0078413486.76473051
CAAGGTC11700.00264123696.75956779
GTCCGAT12300.0043657686.4429931
GTATTTA17902.3932023E-56.42677554
TTCGATT13700.001967716.2985882
AAGAGCA20753.224549E-55.891332
TTCGGTG17200.00408590635.4349732
GAAGAGC18950.00214669155.322541
CGCAGAT18100.00727302865.1744581
CAAAAAC24002.816231E-45.10314371
Produced by FastQC (version 0.11.5)