FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820118
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG550.002635414715.81954630-34
GATCCAA3900.001824825112.9564775
CTCCAAA6403.091729E-612.562521
CTAAAAG4600.00571918111.1225481
CTTACAA5550.001914143610.5356441
CTCCCAT5900.00318008789.9106471
CCTCATT7401.8595235E-49.8771661
CCTCGAA6300.0054654729.2814011
CCCAGAT6450.0066320869.0655541
CTTCGAT9954.100323E-58.8149991
CTCATAT6650.0085194268.7929051
CCCATGC8500.007116217.739051
CCAGATT8450.0075548747.68752052
CAATCTG10900.00119605657.2848344
CTTAAAA10300.0049263397.0962161
CTTTTGT11650.00211980246.90129851
ATCAAAA18303.5738012E-66.78987651
CTTTTAC10800.00779890836.7676881
CCTTCAT11050.0097250516.61457251
CCTTTAT12400.00408078876.48388151
Produced by FastQC (version 0.11.5)