FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410036
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAC5902.0463296E-512.2719081
CTTACGA8851.15094845E-59.8175261
CACCCAG8457.723533E-48.568551
CTCAAAT13603.529094E-78.5181471
CTTCAGA17654.1891326E-98.2044491
GTCCGAA9200.00179135577.87002751
CTATTAT17703.8189683E-87.7722081
ATCTGAG9650.00298088867.472146
CAAGCAT12659.1213085E-57.4407531
CTCAGAT10000.00405461347.2404251
CTTATTT23301.8299033E-97.14720061
CTCATTG12355.61147E-47.03523061
CTTAAAT13401.856239E-47.0242931
CTTCGTA11400.00186512986.98637531
CTTTAAA19752.6152702E-76.96547271
CCTTGAT18858.957959E-76.9139341
CTTATAT18053.350331E-66.81923681
CTTCAAC16354.296696E-56.6425921
CTTTCCG28602.9485818E-96.3290431
CTTCTGT23005.402453E-76.2960221
Produced by FastQC (version 0.11.5)