FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428884
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG3200.00729953313.5696251
CTACCAA9903.8206235E-710.2343311
CTACCGT8951.3222638E-59.703421
CTTCGAA11101.7943323E-58.4759231
CTGCGAT10306.915443E-58.4316121
CTTCGCG9502.6725876E-48.3798391
CTTGGTT9653.1647235E-48.2495821
GTTGACG8350.00663571577.8005031
CTTGGAT9400.0022231937.69907861
CTCGTCA9750.00318091537.42270141
CTTCTAA10800.0010566997.3711551
CTTACTT11450.00196278266.9527051
GTTGAAT14103.482023E-46.67253451
CTTACAA12000.00321531956.6340391
CTCCGAT12200.00382300356.5252851
CTTCGTT14504.8947084E-46.48846441
CTTTGAT25154.2061038E-76.04293441
CTTCCGG26351.5732076E-76.04238841
CTGTATT20401.4521503E-45.6761831
CTTAAAA19504.830238E-45.5670261
Produced by FastQC (version 0.11.5)