FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666048
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG3808.368115E-515.2698611
CCCACAT4000.002128783112.6930731
TTCCCAC4100.00266263712.3202679
CTCCGAT5601.6830285E-411.6569031
CTTCGAC4450.004728853711.4095041
CTCTATT5250.001283457711.0524721
CTCAAGA6003.1923043E-410.8797761
CTTTGAC7652.4453075E-510.4294151
CTTTACT6607.684591E-49.8907061
TAAGCTT6050.0043484599.5420259
CTGAAAC6650.0090685878.7256361
CTTCAAT11001.552577E-58.5719451
CTCATTT8609.02307E-48.4339351
CCTGAAT7950.00417843238.2111521
CTTCCTT13352.3990015E-68.1496461
CTTTAAT10901.3003752E-47.98515751
CTTTGGA9100.00157810037.9705321
CTTCTAT8700.0093781897.50329451
CTTTTAC11000.00125173077.2531841
CTTTGAA13201.5055355E-47.14328771
Produced by FastQC (version 0.11.5)