FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626536
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAC3554.724653E-516.325851
TTACACA4050.004692592714.502031145
CTCCACT3000.004722738614.4891921
CCCGAAT4502.1908732E-514.4891921
TGGTTTA4050.002423581712.4755434
CAAGACA4850.00904599510.4561181
CTCAAGC4900.00975998710.3494221
CTACAAT6658.3242083E-49.8047161
CTCCGGA6850.0010919999.5184471
CTTCAAG6950.0012466969.3814911
CTTCCGT8205.6836713E-48.8348721
CTTTCAT9402.3518616E-48.4777191
CTTCCTT9753.489261E-48.173391
Produced by FastQC (version 0.11.5)