FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501891
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2009.1822585E-932.5326237
ACACGTC2202.3226676E-829.5751138
CACGTCT2559.748692E-825.5106989
AGCACAC2953.9734732E-722.0560155
GAGCACA3208.686693E-720.332894
AACCTAC1950.006948688518.537115
AGAGCAC4054.1719977E-717.8505483
GCACACG3956.494771E-616.4722146
CCTCATT3150.006165973413.9174331
CGAAGGT3300.00838839313.2848221
GAAGAGC5752.0078884E-411.43651
CTTGATT6254.3455616E-410.521581
AAGAGCA7101.3654245E-410.1823552
CAGGAAT6450.0066428069.0625141
ATAAGAT6600.00875080758.7629963
CTTCATT9201.6969487E-48.7362151
CATCAAT10507.0438103E-47.65458871
ACGTCTG2800.00237040316.195455610-14
Produced by FastQC (version 0.11.5)