FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542472
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA6759.405409E-49.6768241
CTCGAAC7450.00231277948.7675931
CCCAAAG10751.1019323E-48.1015271
CTTTCGT13452.6561684E-68.0939981
ACGAGAT10004.7500018E-47.9440443
CTCACGC8550.0079973597.6395981
CTCATGC10557.990543E-47.56718441
CCCGAAT8650.0088728077.55127861
ATCCAAT13151.5199564E-47.1394796
CCTGAAC15401.803192E-57.0691081
GTTTAAA10700.0076415936.78282071
GTTGAAT10850.0087407716.68904921
CTTTAAT19951.5299154E-56.18443631
TAAAGGT14300.0030325736.0603013
CATAAAC14700.00390469455.92458631
CTGAAAT24851.27835265E-55.54908371
CTGGAAT19755.521818E-45.5121151
CTTTCAG26506.4250326E-65.47744751
CTTCTAT18150.0068925845.19829371
CTCGCCG25351.0321357E-45.15333841