FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542673
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG2308.2956854E-418.7956289
TAAAGTG3000.004893698714.4113084
CTTCAAA9151.4878206E-610.306881
ATCAAAG9251.8923074E-59.4111881
CAAAGAA14801.4715624E-88.8229891
GAAAACC7650.00313115268.477242
CAGATTT8250.0061812427.8607133
CATTGAT8350.00648184737.81917671
CTCTTTT13450.00144279996.4723791