FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691487
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAATCTCGTATGCCGT8060.11656039809859044TruSeq Adapter, Index 3 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3350.053.7140739
ACACGTC3650.049.2992178
CACACGT4500.043.1861157
AGAGCAC5150.039.135883
GCACACG5250.035.6456836
AGCACAC5450.034.337585
GAGCACA5650.033.124494
GAAGAGC9100.022.277221
AAGAGCA9750.021.4131472
CTCAGAC3400.012.29262925-29
ACTCAGA3950.010.94585925-29
AGTCACT3400.010.60277620-24
CACTCAG3700.010.51689925-29
GTCACTC4150.09.38153920-24
CTCGTAT4150.09.379535-39
ACGTCTG4500.09.27634710-14
TCAGACG4450.09.06825425-29
TCTCGTA4051.8189894E-128.8991635-39
ACTCCAG5050.08.83612315-19
CAGTCAC4750.08.80365120-24
Produced by FastQC (version 0.11.5)