FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450580
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT8801.0999893E-59.855091
CTCCGAT10751.1572078E-48.0674231
CTTGAAT11703.5334808E-58.0300731
CCCTGTT13402.3263196E-57.5506661
CTCCAAG9950.00393155547.2633831
CCACAAA18704.2060536E-56.18358561
CTTCAAA24703.078767E-76.1444691
CTTCGGA15550.0011583986.04191971
CTCAAAG14550.003650145.9604671
TTCAATT16100.00187117355.80472332
CCCGAAG15450.0071042185.61325551
AAGATTC15650.0087338935.50904565
CTTTCAA21803.8204872E-45.30426841
CTTCAAT20459.322381E-45.30102631
TTGAGAA19250.0026980895.2263273
ATTCCTG19550.00328469845.14470868
CAAAAGA29709.220043E-65.1100471
CAACAAT20000.00404989165.05894571
CCAGCAT21450.00175936455.0538921
Produced by FastQC (version 0.11.5)