FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880617
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGA4954.908478E-513.300591
CCCCTCT4450.004439149511.5072511
CTTCGAA5700.00237268510.2671211
GTTTCGT7301.6075527E-410.0209921
CTCAAAT8104.5682583E-49.0312631
CCCTTTT11602.854622E-68.8288391
CAATGAT15051.706394E-88.7492241
ATCTAAA7600.00257671288.6628841
CTCCCAT8150.0048428038.0782721
CTCATTT11001.3109318E-47.9803531
CTTCAGG8500.0070632597.74563651
CTTCGAT8650.0082596777.6113191
AAGATTC11402.2889912E-47.60542635
CCTGAAT10607.713149E-47.59137341
CTTAAAA12658.016972E-57.5177241
CTTCAAA16901.0081258E-67.35860971
ATAAGAT12901.19084616E-47.2811643
CTTCAAC10450.00562342687.000311
CTCCTTT18002.723189E-66.9089171
CTATAAT11650.00210131436.90717271
Produced by FastQC (version 0.11.5)