FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632149
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTGG3800.00569985114.083599145
CCTAAAA3700.00115104513.7724961
TACACCC3200.0074333313.5307628
CCCAGCT6153.8770694E-410.653291
CTTCAGG7051.1862764E-410.3258841
CTTCGAT5900.00328604469.8708451
CAATGCT6250.005288069.3180771
CAGGATA8350.0067979237.77816254
CTTTATT13251.5074071E-47.1423941
TCAAAGA10200.00506879347.07490732
CTTTGAA11450.00184070466.99364381
CTCCTTT12707.23817E-46.8785021
AATAAAG13300.0096383325.9684565
CAAAGAA15750.008086655.54647451
Produced by FastQC (version 0.11.5)