FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504772
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2603.6379788E-1232.7308049
CACACGT3201.8189894E-1228.8127787
ACACGTC3450.028.777818
GCACACG3609.094947E-1225.611366
GAGCACA3501.70985E-1024.316724
AGAGCAC3552.0190782E-1023.9813583
AGCACAC3957.730705E-1021.5464615
AAGAGCA6755.640959E-712.617422
GAAGAGC7451.3120916E-712.4350921
AAGATAG6050.0050117179.3783495
CTCGTAT2501.1528786E-57.960021535-39
TCTCGTA2457.71931E-57.54229535-39
AGTCACC2908.0589656E-56.87259520-24
ACTCCAG2908.449672E-56.84781215-19
GAGATCT2350.00314377956.648761730-34
CGTCTGA3851.2611559E-56.262901310-14
CTCCAGT3602.148094E-45.910313615-19
AGAGATC3406.505665E-45.84877730-34
CCAGTCA3909.6431504E-55.84044720-24
ACGTCTG3457.9840125E-45.75567610-14
Produced by FastQC (version 0.11.5)