FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675221
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAT7156.201299E-813.1717111
CTCCCTG5300.00140328410.9350051
TAGCTTC4850.00920411910.4318919
CTGAGGA5900.00342151139.822971
CTCAGGA6250.00550490579.2728841
ATCAGCG8004.4439652E-49.0555521
CAACAAC9753.491139E-48.1732151
CAACAAT9853.8963815E-48.0902391
CTTTTGT9700.0029902357.4684961
CTTTAAT10150.00465444347.137381
CAAAAAC10600.00708993156.83437821
AAAGATT14756.079902E-46.3721614
GAATATA13000.00746855756.1176524
CTCTTTT13100.0079193846.08311841
Produced by FastQC (version 0.11.5)