FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10501028
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT64800.07.50197171
CTCAAAT93200.06.46156551
CTTAGAA67050.06.16810371
CTCCAAT82650.06.0573381
CTTTAAT157200.05.8617431
CCCAAAG78450.05.82670741
CTCAAAA143500.05.6629371
CTCCGCT78200.05.47420261
CTTCAAT181150.05.3671291
CTCATGA67650.05.362631
CCCCACT33951.2091732E-75.3428851
CTCAGAT60200.05.3031241
CTCAGTT56200.05.2932591
CTTCAAA212700.05.2873711
CTTCTAT154300.05.2665681
CTCATGT53801.8189894E-125.2596631
CTTAAAT115400.05.21852641
CCCATTT97350.05.21720271
CCTAAAG56200.05.1641551
CTCGCTT52109.094947E-125.1527561
Produced by FastQC (version 0.11.5)