FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533202
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1751.3626413E-732.0230877
ACACGTC2503.0676147E-622.4161578
GAGCACA2405.67053E-520.4352264
CACGTCT3051.7119399E-518.3739019
GCACACG3003.1333847E-416.3481836
AGCACAC3459.056297E-414.215815
AGAGCAC3500.001009729214.0127283
GAAGAGC6004.2614917E-410.5441591
AAGAGCA5850.00416210539.5921482
CCAGAAT9704.5625455E-47.9715291
Produced by FastQC (version 0.11.5)