FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578740
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4400.029.2223327
CACGTCT6000.020.239179
ACACGTC6550.019.6302688
GAGCACA10450.013.6751654
AGCACAC10403.6379788E-1213.0530385
GCACACG10755.456968E-1212.6276546
AGAGCAC10851.1168595E-911.1953593
GAAGAGC19102.2831955E-87.4890791
AAGAGCA20002.9522926E-66.4307462
GGACAAT12850.0074238096.1223951
AGTCACG5251.1944994E-65.71473420-24
CAGTCAC6353.9737643E-85.62473820-24
ATTCTGG17850.00681509365.20334636
Produced by FastQC (version 0.11.5)