FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405201
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT11001.2387318E-911.1277387
ACACGTC12307.807332E-68.1954658
CACGTCT13051.7214605E-57.72457469
GTTCGAA14950.0048786945.80764771
GTTTATT30604.941612E-75.43834351
AGCACAC25552.327032E-55.3544315
ATTCGAA19400.0027339875.22139451
CTTTGAG37453.6079655E-85.2164151
GCACACG23752.4347044E-45.15389976
AATCGAA30953.846948E-65.1181465
TAGATTT25601.358286E-45.06457043
CTTTATT47606.184564E-105.0161111
CTCCGAT24603.8197587E-45.00004631
Produced by FastQC (version 0.11.5)