FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013792
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3755.371403E-616.8080147
ACACGTC3856.898761E-616.3714438
CACGTCT3907.798388E-616.1615529
GCACACG5601.6836622E-512.506586
AGCACAC5902.8748105E-511.8706525
AGAGCAC7452.1835313E-611.2822163
GAGCACA7954.759333E-610.5721214
CTTCAGA12701.3993733E-47.18685151
CTTGAAT13051.9552608E-46.99411
TACATCT11200.00219714326.87827979
AAGAGCA14409.061591E-56.8101462
CTTGTTC11400.00257789346.774651
CTATTAT15802.9376522E-46.22114041
CTTGATT14708.33236E-46.20904871
GAAGAGC12600.0073296096.1294451
GAGTAGG3851.0129472E-45.820957710-14
CGAAGAA17200.00540775345.30657051
CTTTTCT25603.637841E-55.21089941
CTTTGAT20400.00132532285.1625011
CTTTCAT19150.00337711195.13284641
Produced by FastQC (version 0.11.5)