FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981267
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTGG2500.001453762917.2901388
CCCTCAG4201.8729478E-413.8915071
CTAAAGT4600.005815013311.0981071
CTCCGAC4800.00797838310.6356851
CCCATCT8507.333886E-59.4380531
CCTGGAT9000.00134192638.1033791
CTCAAGG8150.00497858378.0536661
CTCGAAA10951.2886745E-47.9923741
CTCATTT10305.864346E-47.78868531
CTCAAAT8450.00688674867.76773641
CATAAAG12708.746714E-57.4653181
CACCAAT10000.00377567627.29304171
CTTGTCG12053.89314E-47.26277971
TCGATAT13251.7140358E-47.0693763
CCTTCAC12606.488726E-46.94575361
CTCTCTT10850.0083331646.7216971
CACAGAA10950.0091041476.66031171
GATTTAT16052.5791727E-46.28405865
CTTCCAT18703.6776917E-56.2400351
CTCTTTT21456.6008106E-66.1200341
Produced by FastQC (version 0.11.5)