FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534149
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAC1850.00519711619.493415
CTCAAAT4551.4029538E-615.8964751
CCCGAAT3200.007319933313.5616811
CTCTTGA5056.5758715E-512.890311
CTCCAAA4800.00847535610.5479751
TTAAAAC5700.002659181610.1257412
TCAAATT6000.0040655699.6194532
ATAAACT6500.0078729938.8773
TGACGAG6600.008997278.7334979
CGCCTTT8750.0011002648.2661681
AGGATTT8250.00612800247.86824945
CTTCTTT12154.694743E-47.1436011
CAAGAAA11800.00270402436.7425311
CCTTTTT12150.00378404726.52989153
Produced by FastQC (version 0.11.5)