FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375019
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCATT11804.127658E-68.59211351
CTTTCGT10256.482033E-58.478351
CTCAAAT10306.861549E-58.4371931
CCCCTTT9502.6533587E-48.38538551
TCATGTT11252.207997E-58.3370932
GATAGAC8500.00773601657.6679191
CCCAAAG9650.0028569967.50458431
CAAAAGG17852.8041995E-66.89707041
CTCCGAT11700.0024472146.80864671
CGTGGAA13300.00130028756.5340671
CTCATAA12700.0057718616.27253251
CCTCGGT13150.0082822826.05788331
CCCAAAA14350.0030562386.05596451
CACCGAT14950.00482403075.8129161
CTTATTT16750.00274559445.62059831
CACAAAG19404.4657916E-45.5994261
CTTCAAT18050.0066252985.21579031
CAAAGAT18150.0070675545.1870531
TTATTGT18350.0084025235.11073976
Produced by FastQC (version 0.11.5)