FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426025
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGTGAAGCATCTCGTATGCCGT4590.10774015609412593TruSeq Adapter, Index 13 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC350.003682710761.635769
CACACGT1558.0035534E-1041.753267
CACGTCT2051.2296368E-831.569549
AGCACAC2401.718945E-929.9653475
ACACGTC2202.4450856E-829.417078
AGAGCAC2508.4441126E-825.890063
GCACACG2951.5525075E-824.3757256
GAGCACA3251.0592175E-619.915434
AAGAGCA5752.3097836E-411.2644822
GAAGAGC5902.8227115E-411.0234171
AGTCACT1502.1155827E-510.56988720-24
GTCACTG2752.6985584E-46.813646320-24
ACTCCAG2450.0042650126.457686415-19
CTCTCGG2600.0063558646.214983125-129
ACTGTGA2550.00637657756.2130525-29
CTCGTAT2950.0043887235.862973735-39
TGAACTC4058.739461E-45.32704815-19
Produced by FastQC (version 0.11.5)