FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637310
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT3650.038.8038441
TCCCTAC3750.031.9752569
TTCCCTA4000.029.9768038
CACTCTT5700.025.9942422
TTTCCCT9600.014.6945117
ACTCTTT9651.8189894E-1213.8896353
CTTTCCC9651.8189894E-1213.8896356
CTCTTTC11953.6379788E-1211.8066494
TCTTTCC13855.456968E-1210.6963135
CGACGCT3003.016794E-88.4640415-19
TACACGA3752.1027518E-87.5235910-14
CCCTACA3301.2346991E-67.26710410-14
ACACGAC2951.47184255E-57.17516662
CCTACAC3401.9768522E-67.05336610-14
CTACACG3801.5765727E-66.68213610-14
GACGCTC5402.5337358E-66.5319196
ACGACGC3551.9143068E-45.96152644
Produced by FastQC (version 0.11.5)