FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3670296
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATT21058.271363E-76.518972
CTTCGAT35551.8189894E-126.5135421
CTCGTAT14856.5565214E-46.33466631
CTAAGAT17158.4080646E-56.32898431
CCCGTGA16252.866882E-46.23421141
CCCCGCT20352.1779551E-56.04493141
GGTAAAT40909.094947E-125.83844851
CCCAAAT22351.4623563E-55.8277621
CTCACAG14950.0048731745.8082721
CTCGGAA19053.5356992E-45.69774631
CTCAAAT28658.021343E-75.5565461
CCTAAAT20800.00115173225.21836951
TACGGAG18350.0083374765.11475669
CATGGAT38306.030132E-85.10119441
CCCACGG21400.00168052475.07205961
CTCCCAT27356.6122724E-55.0269331
Produced by FastQC (version 0.11.5)