FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324545
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTT4805.6594686E-412.1880571
CTCATGT5500.001768957510.6368491
CCCCTGT7702.4054212E-510.4469071
CCCCTTT10608.4388193E-79.6584611
CCTCAAT9402.0889425E-59.3355341
CTTCAAT16901.6007107E-109.0869541
CTCAGGA7400.00203045648.8939881
CTCATTC9753.1609178E-48.2503781
CTCCAAC9903.725857E-48.1253721
CTCAAAG13954.007963E-67.86326271
CTCTTGT9500.00222674767.69772051
CTCAAAT10607.7488384E-47.58879041
CTTCCGA10859.934447E-47.41393381
CTCTCTT10050.00386265397.27645251
CTTCTAT13301.4974934E-47.14788341
CTTTGAT18102.9928124E-66.86840771
CCATAAT13852.4633546E-46.86403231
CTTTATT21701.5744081E-76.73993971
CCCAGAT12000.00288228536.7034311
CCTTGAT19651.5090118E-66.69877961
Produced by FastQC (version 0.11.5)