FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109056
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGT4603.9524914E-412.7182331
CTCACTT6550.0074954588.9318891
CCTCTCC7600.00258538.6601131
CCTACAA9301.8956373E-48.6497671
CCCTGAA8400.0063735157.835341
CTTTTAT26350.07.77091261
CTTCATT18305.761285E-87.59271571
CATCGAT8800.0096612647.4791881
CTTCGTT10809.4521133E-47.448411
TTCGTTC9950.0039569677.25825832
CATCAAT22204.267349E-97.24710131
CTTAAAG10350.00514061757.0656851
CTTCAAG18504.2084903E-66.7200391
CATCCAT16654.811294E-56.5882741
CTTTGAT18402.7911237E-56.35911661
CTTTAAT17801.20968194E-46.16262671
CTTAAAT13500.0098174275.9587281
CTTCTCT16050.00146836835.92328931
CTTTGCT16100.00152407125.90489441
CTTTTAC14950.0043257245.86995361
Produced by FastQC (version 0.11.5)