FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805522
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3400.029.3108489
ACACGTC3256.366463E-1126.2831348
CACACGT3801.8189894E-1124.3522477
GAGCACA4901.0757503E-817.4359384
AGCACAC5353.1872332E-815.9663915
GCACACG6201.2012606E-814.92557056
AGAGCAC5951.171411E-714.3590083
GTGTACC4550.00641901110.9513076
CAAAAGT8951.5320073E-59.5822211
AAGAGCA10751.4574398E-69.2773182
GTAATAA7150.0018290318.9958971
GAAGAGC11402.9304332E-58.1497921
TTCAATC10650.00107585517.35776572
GCCAGAT9800.00377623767.2925921
CAGATTT10000.00477068827.11967473
CCCGGAA11100.00161246487.08235551
TCACACA3602.932254E-56.339147625-29
CAGTCAC4150.00133603815.160529620-24
Produced by FastQC (version 0.11.5)