FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711344
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT5657.76914E-714.2038071
CTTTGTT11452.2149834E-810.1947131
CCCAGAT6200.0048628689.4136661
CAAGGAA11101.6277772E-69.2016471
TTTACCA9854.241147E-58.78928857
CTTATTT11302.0246645E-58.3931581
TACCATA8100.0051984128.0144669
CTTCATT10951.2819235E-47.9951691
CTTTAAT10658.341689E-47.53535271
ACAATTT10100.0046197.143113
ATTTGAA14556.0771243E-57.0198131
TTTTACC11650.00243045376.81201276
TTCATTA10900.0096613826.61884452
CTTTTGT12200.00350809056.5779931
TATTTAA12450.0048734566.37429335
AAGATTT14907.0535846E-46.29456573
CTTTCAT14250.00259690826.14365631
CTTCTTC20458.192236E-45.35128971
CTTTTCT17900.00549817975.29847431
CAAAGAA18750.0094552215.0582771
Produced by FastQC (version 0.11.5)