FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678408
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2251.0808762E-625.2740848
CACACGT2001.2408473E-524.8791777
CACGTCT2858.411556E-619.9532229
GCACACG2750.0030042315.5090966
GAGCACA4700.00825099510.5868834
CTCCGAT5650.002699488810.108111
CTGCCAC8900.00148259998.0211831
GCGGTTA3800.00623252947.8230247145
ATATTGC10700.00115626477.30763536
CAAAAAT17559.104853E-45.6948111
GAATATT15300.00764217655.57516574
CTCGTAT3800.00262870735.23779835-39
Produced by FastQC (version 0.11.5)