FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500363
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2401.8189894E-1235.925137
CACGTCT2950.031.6628259
ACACGTC3153.8198777E-1127.3715278
GCACACG3204.7293724E-1126.9438466
GAGCACA3306.91216E-1126.1325874
AGAGCAC3501.4370016E-1024.6392973
AGCACAC3952.5465852E-1123.646925
GAAGAGC5151.6907507E-816.8056241
AAGAGCA6201.7025559E-713.9231962
CACGCAT3300.00913945713.1134791
AAACTGC5300.001501930410.8453225
CTCGTAT2551.3719837E-68.47719935-39
AGTCACT2702.6752974E-57.472194720-24
ACGTCTG3756.8376976E-66.51520410-14
ACTCCAG3101.6994658E-46.490738415-19
CCAGTCA2905.1688025E-46.45995120-24
CAGTCAC3354.4726388E-46.022365620-24
CACTTCG4603.987343E-65.946270525-29
CTGAACT3908.407946E-55.896112410-14
TCTGAAC3951.0106622E-45.82147810-14
Produced by FastQC (version 0.11.5)