FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736843
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA3250.0083596113.2929475
CTCCGAA6606.887802E-410.0095511
CTCATTT6350.0056304859.2476691
CAACTGG6700.0087455848.7645821
CAGCAAT10550.00596392536.9576661
TCAAAGA12950.00748485476.1165392
GATATTG15350.00687629265.62893535
CTTTATT15950.0084864285.5225111
CAAAAGA16150.0097325945.454121
Produced by FastQC (version 0.11.5)