FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769939
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG1850.005216314519.482314
CTCCCAT5851.2669861E-613.6011911
CTCACGG4755.6764873E-412.1825021
CCTTAAT4500.00524685211.2518941
GTTCGAT4850.00915383110.4399011
CAAATAG5900.0034660429.8079461
CTCCATT9853.971252E-69.5465681
CCACAAA10105.4627126E-69.3102661
CTCCTGA7100.00153735029.1690491
CTTATTC7954.240013E-49.0985671
CTTCAGG7400.00224013538.797331
CTTTCAT13201.5582445E-47.12376451
CCTTGAT10650.00753344046.79188781
AATAATG11700.00257284856.7754195
CAATAAT11750.00258789266.77165651
TCAGAAT10700.0081492266.7368742
CTTTTGC18453.402041E-56.27283241
CTTTTTG19356.805046E-55.98107341
CAGATTT13300.0097550975.961883
Produced by FastQC (version 0.11.5)