FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410150
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAC10306.538212E-79.8407171
CTCGAAT6400.0067317459.0499471
CAACCGA8550.008172577.62100741
GTCAGAT11552.602875E-47.5220331
CGAATAT15251.721173E-57.0924843
CTCTCCG16450.00222220645.72154661
CTTTTGC27101.6718222E-65.61029861
ATCTTGT17150.0038211395.4658316
Produced by FastQC (version 0.11.5)