FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479759
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG6550.0082797458.8248987
GTCAAGT13150.00111443416.62422851
CGAACCA14404.5941904E-46.5229174
CCTCAAC20353.0324554E-66.4207811
CTTCGGT24251.334598E-65.98684551
CTTAAAT21504.805936E-55.7397141
CTCCAAT17909.5023355E-45.67746971
CTTAAAG21302.5831093E-45.45280741
CTTCGAT23351.6718767E-45.28496171
CTCAAAC20000.00383724235.0813351
Produced by FastQC (version 0.11.5)