FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870747
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACA3200.007360927313.5516149
GTAGTAC3300.00902107713.1409598
CTTTAGT4300.00365516711.8105091
CTCAGAT4450.004721907411.4124021
CCCACAT5852.520737E-411.161581
TAACACT4700.007295810610.7644029
AGATCGA4900.00966164310.3643251
CACCAAT8004.382794E-49.0687841
CACAAAT8750.0010691668.2914591
CCCATTT8850.00119644888.1977711
CATTAAT9954.279349E-48.0206331
CTCCAAT8350.00648530677.81978941
CTTATAT13151.717216E-57.72398331
CTCCGGG8550.0080158997.636871
TGTACTT9700.00306158377.45105847
CAAATTA9700.00306502647.4502033
CTTTTCT22452.2972908E-66.1401121
CTTTTAA16754.0829892E-46.06390331
CTTTCAA15600.00114765126.04585551
CGAAAAG14900.0045500515.84297471
Produced by FastQC (version 0.11.5)