FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157603
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC2400.001180312717.8365049
ATAACCC5006.823357E-512.8422838
GTGAGTA3900.001974908412.8217552
GCAGATT16459.595115E-77.38239152
TGAGTAT9900.00424215947.20666843
CTACAAC10050.0047281297.1268111
AAAACCT13101.6831562E-47.08013963
CTTTGAT18507.850267E-76.9688651
CGCAGAT11500.00229164596.85103371
CAGATTC12800.00730886266.13129853
CTTTAAC15450.0078403565.5630641
ACTACCC3750.00212630055.32730735-39
ATTTAAT27902.1194279E-55.114416