FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155687
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCC7150.0016521429.09849
GTACAGT13009.7037724E-46.70544431
GGTGAAT15401.3569476E-46.60384651
GTTCGTT13250.00868218956.0306831
CTCCGGG23254.100066E-65.93636131
CTTCGAT23352.7691802E-55.5998361
CGAGAAT18400.00133518475.52713251
CAACAGA22559.789466E-55.4763611
CAAATGT21703.3312658E-45.35611531
CCTCGAA18050.0063962185.23185631
ACAACCG18250.00771359775.1487163
ATTCGAT18400.0080057215.13233761