FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005011712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005011712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601596
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGA2850.00330833715.28578951
CCTCCAT6354.208567E-511.4342531
CTTCGAG4450.00469132511.4214061
GTTCTAT5109.981728E-411.3894131
CTTATAA8405.96288E-610.3725011
CTTAAAA9602.722547E-69.8322661
CCAATAT7700.00310105348.4865911
TTGGTTT8150.00536081667.98667573
CTTTCTT16559.667929E-87.89688871
TTATAAC8350.00666363447.7953786
CTTTGAT10356.466105E-47.71673971
GTTTCAT8500.0075460047.68785331
CCATCAT8750.0097758867.46821
CTTTGAA10850.00106948337.36112931
CTCTTTT13752.4528155E-46.86470941
CTTTCAA12757.7965256E-46.83364731
TTTAATA16354.3152395E-56.6385212
CTTCTTC18152.5276384E-56.4006611
CAAGAAA12850.00633305036.21542841
CTTGAAA13100.00773374366.0968131
Produced by FastQC (version 0.11.5)